Pasterkamp, Hendrik-Willem (2022) The influence of saccharides on virulent gene expression and collagenolytic activity of Enterococcus faecalis. thesis, Medicine.
Full text available on request.Abstract
Colorectal cancer (CRC) is one of the most diagnosed types of cancer. Standard surgical management is resection of the diseased part of the colon. Continuity is restored by adjoining the remaining ends of the colon, this is called an anastomosis. Leakage of luminal content into the abdominal cavity, anastomotic leakage, is a feared complication. Recently, the involvement of the human gut microbiome on anastomotic leakage has been identified. Some bacteria present in the gut microbiome can express a ‘leak phenotype’, this phenotype consists of the expression of GelE, SprE and Ace and allows them to breakdown collagen. It has been found that a Western-type diet has an important role in the collagenolytic activity of these bacteria. One of the virulent bacteria is Enterococcus faecalis. The aim of this study was to find out what the influence of mono-, di-, tri- and polysaccharides is on the virulent gene expression and collagenolytic activity of E. faecalis. Differences in collagenolytic activity by E. faecalis grown in an environment with different saccharides has been visualized using skim milk agar plates and the accompanying gene expression has been analyzed. This study found that monosaccharides cause an increased growth and virulence of E. faecalis on the short term. Polysaccharides had no influence on the growth of E. faecalis and showed no increased virulence on the short term. However, it did cause a starvation reaction, where collagenolytic activity and virulence was increased on the long term. For CRC patients, these results indicate that limited glucose ingestion before and after surgery could be beneficial in the prevention of anastomotic leakage. Furthermore, these results call for consideration of current fasting policies in CRC surgery.
| Item Type: | Thesis (UNSPECIFIED) |
|---|---|
| Supervisor name: | Hospers, Prof. G.A.P. and Piersma, dr. S. and van Praagh, dr. J.B. and Havenga, dr. K. and Harmsen, dr. H.J.M. |
| Date Deposited: | 14 Jun 2023 11:18 |
| Last Modified: | 14 Jun 2023 11:18 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/3557 |
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