Exter, R.H.S. van (2018) De invloed van stevioside op de groei en zuurproductie van Streptococcus oralis. thesis, Dentistry.
Full text available on request.Abstract
Dental caries continues to be a problem for society due to the increase of soda consumption amongst the youth and an increased risk of root caries in the aging population. Stevioside is a natural sweetener known to be non-cariogenic, although it is not yet recognized to be anticariogenic. There is no scientific knowledge whether adding stevioside to a product with sucrose will delay the caries process. Current research uses in vitro studies to answer the question: Can stevioside delay the growth and acid production of Streptococcus oralis (S. oralis) influenced by sucrose? S. oralis is cultivated in a liquid growth medium with Brain Heart Infusion (BHI) and added stevioside, sucrose or different combinations of these two substances. After 16 hours of growth, the lactate concentration, pH, total bacteria concentration and percentage of dead bacteria of these growth media are measured. Based on the results of a growth curve of S. oralis in these media, it is concluded that the measurements have been performed in the stationary phase of the bacterial growth. The growth media with BHI in which both sucrose and stevioside are added keep a significantly higher pH than the growth media without stevioside. This is confirmed by the results of the lactate concentration, which is lower in the growth media with added stevioside. Furthermore, the total concentration of bacteria remains lower and the percentage of dead bacteria is higher in growth media where stevioside is added in addition to sucrose. Therefore, it can be concluded that S. oralis produces less acid and grows slower by adding stevioside to a growth medium with BHI and sucrose. This extension of initial research with Streptococcus mutans is a step closer to the in vitro evidence that stevioside possesses anticariogenic properties. This in vitro research can be expanded with further studies on different glucoside compounds of Stevia. Moreover, the initial research design can be made more realistic by applying a biofilm, including a salivary flow and using lower concentrations of Stevia. Finally, the anticariogenity of Stevia has to be investigated in vivo to state the clinical value of the sweetener as an addition to a modern diet.
| Item Type: | Thesis (UNSPECIFIED) |
|---|---|
| Supervisor name: | Hoogmoed, dr. C.G. van and Ma, dr. M.S. |
| Faculty: | Medical Sciences |
| Date Deposited: | 23 Nov 2021 13:28 |
| Last Modified: | 23 Nov 2021 13:28 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/2838 |
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