Beems, B. (Bas) (2018) The effect of dentine matrix components on the mineralization capacity of human mesenchymal stem cells. thesis, Dentistry.
Full text available on request.Abstract
In teeth, dentin matrix orchestrates hard tissue repair through the release of dentin extracellular matrix components (dEMCs) by regulating the differentiation of resident mesenchymal stem cells (MSCs). However, little is known on the effect of dEMCs on other MSCs populations, with the MSCs origin likely bearing an effect on the dEMCs-induced differentiation and mineralization. This thesis investigates the effect of human dEMCs on basic cellular functions and mineralization capacity of human MSCs. Results showed that dEMCs in osteogenic cultures of human umbilical cord mesenchymal stem cells (UC-MSCs) and dental pulp stem cells (DPSCs) enhanced their mineralization, while adipose stem cells (ASCs) were barely affected. In fact, UC-MSCs showed a higher baseline mineralization capacity than ASCs and DPSCs. UC-MSCs and DPSCs showed a dose-dependent viability and proliferation, whereas proliferation of ASCs remained unaffected. Qualitative analysis of the dEMCs-supplemented osteogenic cultures through scanning electron microscopy demonstrated differences in the architecture of the deposited mineralized structures. Large�sized mineral accretions upon a poorly organized collagen network was the prominent feature of UC-MSCs osteogenic cultures, while mineral nodules interspersed throughout a collagen mesh characterized the respective DPSCs cultures. Overall, the stem-cell dependent action of dEMCs on the mineralization of various human MSCs types and the influence of stem cell origin on the architecture of the generated mineralized tissue has been highlighted. UC-MSCs under the guidance of dEMCs appear as a promising regime for craniofacial bone tissue engineering.
| Item Type: | Thesis (UNSPECIFIED) |
|---|---|
| Supervisor name: | Sluis, dr. L. van der (Luc) and Petridis, X. (Xenos) |
| Faculty: | Medical Sciences |
| Date Deposited: | 23 Nov 2021 13:10 |
| Last Modified: | 23 Nov 2021 13:10 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/2834 |
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