Breast cancer microenvironment – and its role in tumor migration.

Roya, S. (Shahira) (2015) Breast cancer microenvironment – and its role in tumor migration. thesis, Medicine.

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Abstract

Breast cancer tumor microenvironment (TME) is increasing in popularity in studies in tumor migration and metastasis. Adipocytes and fibroblasts are such TME cells that excrete several factors, such as SDF-1, inducing tumor migration and metastasis. The present study is a subproject of a study that focuses on factors secreted by adipose tissue affecting migration of breast cancer cells. By conducting xCelligence migration assays we found that migration under influence of adipose tissue supernatant is breast cancer cell line and patient-specific. Fractionation of culture medium, which forms the background of adipose tissue supernatant, was done with ultrafiltration devices and showed cell line-specific effect on cell viability. This might influence future migration assays in which the effect of fractionated supernatant on cell migration will be studied. Moreover, for the first time we studied the expression of SDF-1 in TMAs of male breast cancer patients by means of immunohistochemistry and found that SDF-1 expression is not a predictor for overall survival (OS), but it is related with tumor progression in other ways. Future studies are needed in finding the specific factors that are responsible for migration under influence of adipose tissue supernatant and to reveal mechanisms behind the role of SDF-1 expression in clinical outcome in male breast cancer patients.

Item Type:	Thesis (Thesis)
Supervisor name:	Supervisors: and Nienhuis, H. H. and Timmer-Bosscha, H. and Schröder, C.P. and Department of Medical Oncology and University Medical Center Groningen
Faculty:	Medical Sciences
Date Deposited:	25 Jun 2020 11:01
Last Modified:	25 Jun 2020 11:01
URI:	https://umcg.studenttheses.ub.rug.nl/id/eprint/2095

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