Cleveringa, Annebeth (2012) Het effect van alcohol op de darmwandintegriteit, vastgesteld met I-FABP, L-FABP en LBP. thesis, Medicine.
Full text available on request.Abstract
Objective: The aim of this pilot study is to determine the immediate effects of oral alcohol consumption on gut wall integrity in healthy male volunteers. The gut wall integrity will be assessed using intestinal- and liver-fatty acid binding proteins (I-FABP and L-FABP) as a measure of the integrity of the enterocyt. Lipopolysaccharide Binding Protein (LBP), a protein that binds lipopolysaccharides (LPS), will serve as a measure of endotoxemia. Methods: A clinical trial in randomized cross-over design, including fifteen healthy adult male volunteers. Blood samples were collected at seven specified moments at two different days. On one day alcohol was consumed (1g/kg), the other day water was consumed. Both after fasting 6 hours to obtain a reproducible alcohol uptake. Concentrations I-FABP, L-FABP and LBP in blood plasma were analyzed using ELISA. Results: There were 15 males, median age 21 years [18-41]. IFABPmean (ng/ml), mean of all samples per person, is significantly higher after drinking alcohol than after drinking water, 1,64 [0,81-5,63] vs. 1,15 [0,52-1,81] resp.; p=0.001. Median of the maximum is also higher, 3,16 [1,31-13,29] vs. 1,80 [0,77-2,68] resp.; p=0,001. L-FABPmean (ng/ml) is significantly higher in the alcoholgroup, 18,99 [9,25-37,19] vs. 16,16 [10,52-30,52]; p=0,012. As well as the maxima of L-FABP, 31,70 [13,90-73,72] vs. 24,24 [16,98-36,80]; p=0.009. LBPmean (µg/ml) shows no significant difference, alcohol 15,13 [9,44 – 23,16] vs. 15,74 [9,20-41,39] resp.; p=0.394. LBP maxima are not significant, alcohol 20,50 [11,28-28,60] vs. water 20,32 [11,98-48,28]; p=0.570. Correlations between I-FABP en L-FABP levels are strongly positive significant (ρ=0.843; p=0.000), as well as correlations between maxima of I-FABP and L-FABP (ρ=0,843; p=0,000). Correlations between LBP and I-FABP (p=0.033, 0,148) and LBP and L-FABP are weak (0,209, p=0.003). Maximum concentrations LBP do not significantly correlate with maxima of I-FABP/L-FABP. (0,479, p=0.071/0,211, p=0.451). I-FABP and L-FABP concentrations increase immediately after drinking alcohol. Highest concentrations are measured 3 hours after alcohol intake, whereafter concentrations slowly decrease. Conclusions: Acute alcohol consumption immediately effects the gut wall integrity, as measured with damage markers I-FABP and L-FABP, there seems to be superficial cell damage. It can not be proved or disproved that the gut becomes permeable for bacteria and micro-bacterial products based on the results, LBP concentrations do not increase significantly. As this is a pilot study, population size is small and more research is needed to confirm the outcomes of this study.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Facultair begeleider: and Hulscher, JBF Kinderchirurg and Afdeling: Chirurgie and UMC Groningen |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:40 |
| Last Modified: | 25 Jun 2020 10:40 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/209 |
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