Kuipers, M (Michiel) (2012) A New Model for Isolated Dual Perfusion of Rat Livers. thesis, Medicine.
Full text available on request.Abstract
Liver transplantation is a successful treatment of end stage liver disease. The preservation method currently used to protect the graft between procurement and implantation is simple cold storage (SCS). SCS provides sufficient protection for high quality grafts but not for lower quality grafts that come from extended criteria donors (ECD). Machine perfusion is a promising preservation technique that can possibly protect the liver from ischemia, give the surgeon a better idea of its function and provide a platform to improve the function of the liver. We have built a perfusion machine for rat livers that is able to normothermically perfuse via both the hepatic artery and the portal vein (Isolated dual Perfused Rat Liver; IdPRL). A start was made in identifying a perfusion fluid that can be used as a basis for further research done on this machine and which maintains a functional liver for at least 120 minutes. It is important that such a research model for rat livers was created because it is necessary to improve machine perfusion techniques which can be used for the perfusion of human livers in the future. The IdPRL can also be used as a reperfusion model eliminating the need to transplant a rat liver. Furthermore, research into liver functions can be done without the interference of the natural regulatory systems inside a living animal. The system is able to perfuse at 37 degrees Celsius and is pressure controlled. This paper will discuss how this machine was constructed and what can be done as follow up research to find a right perfusion fluid.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Facultair begeleider: and Porte Prof. Dr. R.J. and Dagelijks begeleider: and Dries Mw. S. op den and Locatie: chirurgisch onderzoekslaboratorium UMCG |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:40 |
| Last Modified: | 25 Jun 2020 10:40 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/199 |
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