Determining the prognostic value of Mesothelin in epithelial ovarian cancer treated with current standard treatment.

Rasmijn, D.D.D. (Donn) (2014) Determining the prognostic value of Mesothelin in epithelial ovarian cancer treated with current standard treatment. thesis, Medicine.

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Abstract

Epithelial ovarian cancer (EOC) is not only the most frequent cause of gynecological cancer death in the United States, but also in the Netherlands (1,2,3). Recent research outcomes have demonstrated a potential tumor associated antigen (TAA), called mesothelin (MSLN), which was overexpressed on a considerable amount of epithelial ovarian cancer tissues and having a restricted or limited expression in normal cells (36,37,41). MSLN was shown to promote dissemination of ovarian cancer to the peritoneum, carcinogenesis and was correlated with a decrease in survival in multiple studies (43,44,46,48). However, still little is known about MSLN expression and survival on a cellular level in EOC. Immunohistochemical staining was therefore performed on a serous TissueMicroArray (TMA), containing ovarian and omental tissues and consisting of a well-defined cohort of patients receiving standard treatment. Tissues were scored and categorized into no MSLN expression, low, moderate and high MSLN expression and were afterwards dichotomized into MSLN expression (low, moderate, high) and no-expression. As a result, about 66% of tissues were positive for MSLN, showing consistency with previous research outcomes. Furthermore, no significant difference between ovarian and omental staining were noted, implicating similar MLSN intensity in omental metastases. Comparing interval tissues and primary tissues resulted in no significant difference in MSLN staining outcomes, implicating minimal/no influence of chemotherapy on membrane MSLN expression. Most importantly, our study did not found a correlation between MSLN expression and a decrease in survival, even after testing for possible differences in clinicopathological characteristics that might exist between groups.

Item Type:	Thesis (Thesis)
Supervisor name:	Nijman, H.W. and Duiker, E.W. and Wisman, GBA
Faculty:	Medical Sciences
Date Deposited:	25 Jun 2020 10:56
Last Modified:	25 Jun 2020 10:56
URI:	https://umcg.studenttheses.ub.rug.nl/id/eprint/1627

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