Mubarak-Gabrian, M. (Mariam) (2017) Targeted Gold Nanoparticles for the Computed Tomography Detection of Breast Cancer. thesis, Medicine.
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Abstract
Introduction: As a response to the need for earlier detection of malignant tissues and metastases, functional imaging has been gaining interest over structural imaging. Gold has proven to be an attractive alternative to conventional contrast agents, with a higher atomic number and a higher absorption coefficient compared to iodine. Due to these properties, gold nanoparticles (AuNPs) could offer a 3-fold increase in contrast per unit weight compared to iodine-based contrast agents, as well as a better safety profile compared to iodine or Gadolinium-based agents in terms of renal and cutaneous toxicity. Methods: Breast cancer cells MDA-MB-231, macrophages RAW 264.7 and fibroblasts MC 3T3.E1 were scanned using a pre-clinical CT to analyze gold nanoparticle uptake. The cells were analyzed after incubation with a AuNPs solution for four hours and silver staining using light microscopy. Results: Breast cancer cells showed the most intense signal for 2-DG-AuNPs (916 HU), and 2-NH2-DG-AuNPs (435 HU), with almost no signal registered for the bare AuNPs, at 22 HU as opposed to 19 HU for the control sample. Conclusion: Functionalized 2-DG-AuNPs were a valid targeting contrast agent for cell lines MDA-MB-231 and RAW 264.7, exhibiting high radiointensity upon CT imaging and low toxicity.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Faculty Supervisor: and Jansen, Liesbeth MD and Oncology, University Medical Center Groningen |
| Supervisor name: | Secondary Supervisor: and Danila, Delia PhD and Medicine, Division of Cardiology and Center at Houston, Texas |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:55 |
| Last Modified: | 25 Jun 2020 10:55 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/1563 |
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