A comparison of the cellular and inflammatory responses between immortalized, telomere shortened and primary airway cell models to HRV1B.

Bos, F.J. van den (Férence Joseph) (2013) A comparison of the cellular and inflammatory responses between immortalized, telomere shortened and primary airway cell models to HRV1B. thesis, Medicine.

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Abstract

Background: The cellular and inflammatory responses in airway epithelial cells in cystic fibrosis (CF) is being investigated in the study of CF pathophysiology. Human rhinovirus (HRV) is an important trigger of exacerbations in cystic fibrosis (CF). Either cell lines or primary cell cultures can be used to study the response of CF epithelial cells in vitro. The use of cell models has an advantage over primary cell models, as primary cells are difficult to obtain. However, uncertainty exists about how accurately the inflammatory response of cell models reflect that of primary cell cultures. Aims: To investigate if cell models have similar cellular and inflammatory responses to HRV compared to primary cells and to determine which of the cell models assessed closely resembles primary cell models. Methods: This investigation compared the cellular and inflammatory responses to HRV in healthy and CF immortalized and telomere shortened epithelial cell models to the responses found in their primary cell counterparts. Cytopathic effect, susceptibility to HRV1B, viability, apoptotic response and inflammatory cytokine production (IL-8 and IL-6) were determined under basal conditions and after HRV1B infection. Results: Similarities were found between the primary cells and cell models when investigating the cytopathic effect (CPE). Furthermore, HRV1B had a comparable effect on the viability among the primary cells, NuLi-1’s and CuFi-1’s. However, when the susceptibility to HRV1B was compared it showed that except for the BEAS2B-s, all of the cell models had a minimal response compared to the primary cells. Furthermore, both the generated CF airway epithelial cell models expressed a lower apoptotic response and appeared to have lower cytokine productions (IL-8 and IL-6) in response to HRV1B. Conclusion: Generated cell models differ in their cellular and inflammatory responses compared to that of their primary counterparts. Furthermore this study demonstrated that the telomere shortened cell models reflected more truly the cellular and inflammatory response of primary cells to HRV1B infection compared to immortalised cell models.

Item Type:	Thesis (Thesis)
Supervisor name:	Brand, Dr. P.
Supervisor name:	Schultz, Dr. A. and Princess Margaret Hospital for Children and Perth, Australia
Faculty:	Medical Sciences
Date Deposited:	25 Jun 2020 10:50
Last Modified:	25 Jun 2020 10:50
URI:	https://umcg.studenttheses.ub.rug.nl/id/eprint/1144

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