Dijkstra, B.M. (Bianca) (2017) Establishment and characterization of in vitro Establishment and characterization of in vitrofluorescence-assisted resection. thesis, Medicine.
Full text available on request.Abstract
BACKGROUND: Meningiomas are the most frequently occurring primary brain tumors in adults and are usually benign. Surgical removal can only be curative with a complete resection and thus, several intraoperative techniques have been introduced to improve surgical resection, including intraoperative fluorescent imaging. Somatostatin receptor subtype 2 (SSTR2) is a meningioma-specific biomarker that may be used as a novel fluorescent imaging marker. We aimed to generate and characterize new patient-derived meningioma cell cultures as preclinical models for exploring the possible value of SSTR2 expression-guided surgery. MATERIAL AND METHODS: For the generation of meningioma spheres, tumor tissue was processed and seeded in supplemented sphere medium. For differentiation, cells were exposed to serum-containing medium and differentiation was examined by immunohistochemistry (IHC) for presence of β3-tubulin, Vimentin, glial fibrillary acidic protein (GFAP) and epithelial membrane antigen (EMA). SSTR2 expression was determined on primary tumor material, spheres and differentiated meningioma cells using IHC. RESULTS: Eleven new sphere forming meningioma cultures were established of which three were characterized in more detail. Spheres were positive for vimentin and EMA, but negative for GFAP. Additionally, differentiated meningioma cells were positive for β3-tubulin. All primary meningioma material stained positive for SSTR2, whereas two out of two meningioma spheres and all differentiated cells were SSTR2 positive. CONCLUSION: Meningiomas spheres were successfully generated and characterized. They demonstrated stem cell properties based on differentiation ability and associated marker expression. The meningioma spheres and SSTR2 appear to be suitable tools for further preclinical development of a new intraoperative fluorescence guided imaging approach.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Department of Medical Oncology, UMC Groningen and Faculty supervisor: and Kruyt, prof. F.A.E. and Department of Neurosurgery, UMC Groningen and Second supervisor: and Groen, prof. R.J.M. |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:48 |
| Last Modified: | 25 Jun 2020 10:48 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/966 |
Actions (login required)
 |
View Item |