Beesems, G. (Geerte) (2016) Changes in the Gut Mycome and Microbiome during Necrotizing Enterocolitis-like Murine Intestinal Injury. thesis, Medicine.
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Abstract
INTRODUCTION – Necrotizing enterocolitis (NEC) is a gastrointestinal crisis in the newborn infant. NEC is among the most common and devastating diseases in newborns with a mortality up to 30% and still one of the most difficult to eradicate. NEC is therefore a priority for research. Unfortunately, the pathophysiology of NEC is not completely understood. A multifactorial cause is suggested in previous research, most investigative attention has remained focused on the role of bacterial flora, and increasing evidence indicates that luminal fungi may also play a role. In this study, the difference in the microbiome and mycobiome have been monitored using a proven mouse model to establish the best techniques for detecting disease progression in treated versus control mice. MATERIAL AND METHODS – A research set up was designed and primer pairs selected. Ten day old mouse pups were used to mimic NEC and the gut including feces was harvested for DNA extraction. The DNA was then used for next generation sequencing in the Miseq. The DNA sequences were analyzed using BaseSpace and QIIME. RESULTS – The research showed some obvious differences in the microbiome between the control versus the treated group. Where some species were only prevalent in the Control group and other species only prevalent in the Treated group. Due to a low number of reads off the mycobiome in the samples, no differences in the mycobiome could be detected. CONCLUSION AND DISCUSSION – Some of the results of this research are also found in previous research. This could indicate the pathological and/or preventive function of several species. The mycobiome was inconclusive and more research needs to be done on this topic.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Faculty supervisor: and Buist, Girbe |
| Supervisor name: | Deschenes, Dr. Robert and Maheshwari, Dr. Akhil and University of South Florida ǀ Morsani College of Medicine |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:48 |
| Last Modified: | 25 Jun 2020 10:48 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/923 |
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