Angremont, G. d' (Guido) (2014) Op zoek naar de effecten van sigarettenrook op de integriteit en permeabiliteit van darmepitheel. thesis, Medicine.
![[img]](https://umcg.studenttheses.ub.rug.nl/style/images/fileicons/text.png) |
Text
AngremontdG.pdf Restricted to Registered users only Download (944kB) |
Abstract
Inflammatory bowel disease (IBD) is characterized by recurrent inflammations of the gastrointestinal tract and consists of Ulcerative colitis (UC) and Crohn’s disease (CD). To this day the exact pathological process is unknown, although there seems to be a disturbance of the barrier in the gut in IBD patients. Some studies show this might be due to a change in the tight junctions between epithelial cells. One of the environmental factors that influences the disease course of IBD is smoking. Smoking appears to be a risk factor for the development of CD and on the contrary seems to be protective for UC. In the current study we hypothesize that smoking, or one of the main smoke components nicotine and carbon monoxide, influences the integrity and permeability of the gut wall via the tight junctions and by that means is capable of changing the susceptibility for IBD. In this experiment we make us of a newly designed model from found in literature where a cell line grows in a three dimensional cell culture which gives them the opportunity to polarize into monolayer spheres with a lumen. Materials and methods The human intestinal epithelial cell line Caco-2 was seeded out single cell in a solution of medium and Matrigel. In 7 days the Caco-2 cells grew into 3D spheres. Cigarette smoke extract (CSE), nicotine or CO was added from day 0 or on day 7. The cytokine TNF-α was used as a model for inflammation. Effects on the tight junctions were assessed by the permeability with the use of FITC-dextran and fluorescent microscopy, by the RNA expression with the use of PCR and by the protein expression with the use of Western blotting. Results Smoke and it’s components seem to have little or no effect on the expression of the tight junctions measured in this study. Neither does cigarette smoke seem to have a protective effect against increasing permeability of the epithelial layer induced by TNF-α. We did find that cigarette smoke extract has a disturbing effect on the morphogenesis of the epithelial cells. Conclusion Determent by our experiment smoking does not seem to exhibit it’s positive effect on UC by modulating the expression of tight junctions in the epithelial layer of the gut. On the other hand smokings detrimental effect on CD seems to be partially explained by our observed perturbation of the morphogenesis of epithelial cells and this requires more thorough research.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Facultair begeleider: and Dijkstra, prof.dr. G. and Dagelijkse begeleiding: and Blokzijl, ing. T. and Maag-Darm-Leverafdeling research laboratorium and Universitair Medisch Centrum Groningen |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:47 |
| Last Modified: | 25 Jun 2020 10:47 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/862 |
Actions (login required)
 |
View Item |