Non-Anastomotic Biliary Strictures After Liver Transplantation: Injury, Regeneration and Preservation of the Biliary Tree.

Weeder, P.D. (Pepijn) (2014) Non-Anastomotic Biliary Strictures After Liver Transplantation: Injury, Regeneration and Preservation of the Biliary Tree. thesis, Medicine.

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Abstract

Non-anastomotic biliary strictures (NAS) are a feared complication causing significant morbity and mortality in liver transplant recipients. The etiology of NAS is not fully understood, although ischemia is believed to be a major risk factor for biliary injury. In this study, injury of the peribiliary vascular plexus and deep peribiliary glands (dPBGs) in donor bile ducts at the end of cold storage were strongly associated with donor risk factors. Injury of the dPBGs is an independent risk factor for the development of NAS after transplantation. A method for the quantification of biliopancreatic progenitor cells in the PBGs was developed and a proof-of-concept was achieved in a pilot study. Secondly, a putative mouse model to study the role of biliopancreatic progenitor cells in biliary regeneration after injury was presented. Finally, a new bile duct model for the comparison of different static preservation solutions was designed. This model was applied to assess the effect of oxygen (carrier) enrichment of University of Wisconsin preservation solution. However, because of high variability and relatively low power, the results were inconclusive. Overall, the work presented in this thesis may contribute to a better understanding of injury, regeneration and preservation of the biliary tree in the liver transplant patient.

Item Type:	Thesis (Thesis)
Supervisor name:	Porte, Prof. Dr. R.J.
Supervisor name:	Uygun, Prof. Korkut PhD and Harvard Medical School
Faculty:	Medical Sciences
Date Deposited:	25 Jun 2020 10:47
Last Modified:	25 Jun 2020 10:47
URI:	https://umcg.studenttheses.ub.rug.nl/id/eprint/803

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