Ganeshamoorthy, S. (Steby) (2019) Change in C5L2 mRNA expression in response to a high fat diet and the role of antidiabetics on C5L2 receptor expression. thesis, Medicine.
Full text available on request.Abstract
A novel finding is the involvement of the C5L2 receptor in metabolism. Upon dietary intake, triglycerides are packed into chylomicrons and transported to tissues for energy utilisation and storage. The chylomicrons bind to C3 molecule thereby generating ASP (acylation-stimulating protein) which binds to the C5L2 receptor leading to triglyceride uptake and storage. For the first time, this study assesses the changes in C5L2 mRNA expression in response to a high fat diet in parallel with exercise regime as well as investigating the modulatory influence of antidiabetics on C5L2 receptor expression. Mice were allocated into different groups each receiving either a high-fat or normal diet with or without exercise after which the organs were harvested and prepared to extract the RNA for cDNA synthesis and qPCR. The second part of the project concerned in-vitro studies in which a mouse liver cell line was grown in culture, stimulated and assessed for C5L2 expression using qPCR. The expression of C5L2 mRNA was found to be significantly higher (2.75 fold) in the high-fat diet fed mice. In-vitro stimulations showed that expression of C5L2 is time-dependent with highest expression (1.79 fold) observed at 24 hours. Under the treatment with Metformin, the expression of C5L2 was upregulated to 2.27 fold in the cultured hepatocytes. We concluded that C5L2 plays a role in the handling of a nutrient rich diet. Metformin and FXR mimetics may play a beneficial role in aiding the function of the C5L2 receptor.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Faculty supervisor: and Bergink, Dr. Steven PhD |
| Supervisor name: | Local supervisor: and Stover, Dr. Cordula MD and University of Leicester, Leicester |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:44 |
| Last Modified: | 25 Jun 2020 10:44 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/542 |
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