Validation of proteins found by proteomics to distinguish between germinal centers derived and activated B-cell type Diffuse Large B-cell Lymphoma

Alsagoor, Y. (Yasir) (2017) Validation of proteins found by proteomics to distinguish between germinal centers derived and activated B-cell type Diffuse Large B-cell Lymphoma. thesis, Medicine.

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Abstract

Background Diffuse large B-cell lymphoma (DLBCL) is the most common and aggressive non- Hodgkin lymphoma. Gene expression profiling has subdivided DLBCL into germinal center B-cell (GCB) and activated B-cell (ABC). Loss of HLA in lymphoma may inhibit anti-tumor response and is associated with a poor prognosis. A protein profile was made of primary tumor cells of GCB and ABC subtypes DLBCL and of patients with and without HLA expression using proteomics. We aim in this study to validate the proteins with differential expression in distinct DLBCL subtypes and to investigate if there are differences in immunological response proteins after loss of HLA in DLBCL. Methods and results Protein expression immunohistochemistry was conducted on 63 cases, of which 31 ABC’s and 32 GCB’s, 36 with HLA class I positive, 27 with HLA class I negative, 46 with HLA class II positive, 17 with HLA class II negative, 23 with double HLA positive and 4 with double HLA negative. The expression of ADK and GLMN were significantly higher in GCB, while ARMC6 and CSNK2A2 were significantly elevated in ABC. Our analysis also showed a significant higher expression for MAVS in HLA negative patients. HMOX was not significantly higher expressed in HLA class I and II negative patients, whereas a trend was noticed in double HLA negative patients. The expression of PPM1A was not significantly elevated in the negative cases for HLA class I and double HLA, but significantly elevated in HLA class II. Finally, EIF4G1 expression was not significant elevated in the positive of HLA class I and double HLA, but significantly elevated in HLA class II positive cases. Conclusion Our research study revealed that the proteins were up or down regulated in the DLBCL subtypes and in cases with loss of HLA expression. We could for the first time validate our proteomics data.

Item Type:	Thesis (Thesis)
Supervisor name:	Visser, Dr. Lydia and Pathology Department, UMCG and Groningen, The Netherlands
Faculty:	Medical Sciences
Date Deposited:	25 Jun 2020 10:43
Last Modified:	25 Jun 2020 10:43
URI:	https://umcg.studenttheses.ub.rug.nl/id/eprint/460

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