G. J. Julia, Huisman (2024) Urinary Mitochondrial DNA predicts Renal Allograft Function in Living Donor Kidney Transplantation. thesis, Medicine.
Full text available on request.Abstract
Background. Complications and impaired kidney function frequently arise after kidney transplantation due to ischemia reperfusion injury (IRI). Mitochondrial DNA (mtDNA) implicated in organ damage caused by IRI, has shown promise as a biomarker for assessing and predicting graft function. This study aimed to explore the presence, dynamics, and prognostic value of urinary mtDNA in a cohort of living donor kidney transplant (LDKT) recipients. Methods. This study is a retrospective analysis of the VAPOR-1 study consisting of 57 donor-recipient couples in an LDKT cohort. Urinary mtDNA was measured in the first produced urine, at 2 hours post-operative and on days 1, 2, and 6 after transplantation. Three mtDNA genes were considered, consisting of ND1, ND6, and D-loop. Results. In the first urine after transplantation, levels of mtDNA were associated with graft function, defined by estimated glomerular filtration rate (eGFR), at 6 and 12 months. Higher first urine mtDNA levels were associated with higher eGFR, suggesting that first urine mtDNA levels reflect renal function rather than renal injury. Furthermore, donors and recipients receiving sevoflurane-based anesthesia had higher levels of mtDNA in the first urine, which showed a trend towards better graft outcome at 3 and 24 months after transplantation. Conclusion. This study underscores the potential of urinary mtDNA as a prognostic tool for renal allograft function and advocates a re-evaluation of the way early post-transplantation renal damage markers are interpreted.
| Item Type: | Thesis (UNSPECIFIED) |
|---|---|
| Supervisor name: | Nieuwenhuijs-Moeke, G. J. |
| Faculty: | Medical Sciences |
| Date Deposited: | 04 Oct 2024 11:43 |
| Last Modified: | 04 Oct 2024 11:43 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/3763 |
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