van Hoorn, E.G.M. (Eline) (2020) Placental macrophage polarization in intrahepatic cholestasis of pregnancy: an inflammatory switch correlated to clinical parameters. thesis, Medicine.
Full text available on request.Abstract
can lead to complications for both mother and fetus. Immunological adaptations in placental tissue, and specifically inflammatory (M1 subset) or anti-inflammatory (M2 subset) macrophage polarization have been associated with various pregnancy disorders. However, the influence of ICP on macrophage numbers and polarization remains unknown. This study aimed to analyze macrophage density and distribution in the placentas of patients with pregnancies complicated by ICP compared to control placentas. Secondarily, it is aimed to correlate clinical parameters to macrophage distribution and ursodeoxycholic acid (UDCA) use. Materials and methods: An observational unmatched case-control laboratory study on 42 placental tissue samples of women diagnosed with ICP and 50 controls placentas was conducted. Immunohistochemical staining was performed on the placental coupes using CD68 antibody as a pan-macrophage marker and CD206 antibody as a M2 macrophage marker. Macrophage density (cells/mm2) and distribution (CD206/CD68) in decidual tissue and villous parenchyma were determined and compared between ICP and control groups. Immunological data of ICP patients were correlated to clinical parameters. Results: The density of CD68 and CD206 macrophages did not differ significantly between patients with ICP compared to controls in both decidual tissue and villous parenchyma. However, in villous parenchyma the CD206/CD68 ratio was significantly lower in patients with ICP compared to controls (p=0.047). No difference was found based on UDCA use. Significant weak and moderate correlations were found between macrophage density and distribution and peak serum bile acids and liver enzymes. Conclusion: In the villous parenchyma of women with ICP there seems to be a shift towards a more inflammatory environment, indicated by a lower CD206/CD68 ratio. ICP seems to affect placental tissue, but more research is required.
| Item Type: | Thesis (UNSPECIFIED) |
|---|---|
| Supervisor name: | Dr. Prins, JR and Prof. dr. van Goor, H |
| Faculty: | Medical Sciences |
| Date Deposited: | 24 Oct 2023 11:18 |
| Last Modified: | 24 Oct 2023 11:18 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/3712 |
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