Horníková, Zuzana (2022) Collagenolytic bacteria are present in patients that develop anastomotic leakage after esophagectomy. thesis, Medicine.
Full text available on request.Abstract
Background: Anastomotic leakage (AL) is the most dreaded complication after esophagectomy. It can result in morbidity, reintervention, and mortality. The pathophysiology behind this complication is not completely understood. Recent studies show that gastrointestinal microbiota play a role in the development of AL after colorectal resection via collagen degradation. This exploratory study aimed to establish whether bacteria also play a role in AL after esophageal resection. Therefore, the core esophageal microbiome of esophageal carcinoma patients undergoing esophagectomy was demonstrated, and the association of collagenolytic bacteria with the development of AL after esophagectomy was investigated. Methods: Swabs from the anastomotic sites of esophageal carcinoma patients undergoing esophagectomy were taken. They were cultured and sequenced using 16S ribosomal RNA gene sequencing in order to establish the bacterial present. Shannon index and Principal Coordination Analysis of Bray-Curtis Dissimilarity Index were used to assess microbial diversity. Bacterial strains isolated from culturing were tested for their collagenolytic activity using skim-milk plates. Results: Ten patients were included in this prospective observational study. Sequencing showed that Streptococcus, Prevotella, Gemella and Veillonella were the most abundant genera of patients undergoing esophagectomy. Alpha and beta diversity of anastomotic leakage samples was significantly different from no leakage samples (p=0.0374 and p<0.001). Skim-milk plate cultures showed that several strains of different species, Pseudomonas aeruginosa, Bacillus cereus and several Staphylococcus species among others, have collagenolytic abilities. These bacteria were cultured from leakage samples. Conclusion: Anastomotic leakage samples have shown to have more collagenolytic bacteria and less microbial diversity than control samples.
| Item Type: | Thesis (UNSPECIFIED) |
|---|---|
| Supervisor name: | Olinga, Prof. dr. P. and Haveman, Dr. J.W. and Harmsen, Dr. ir. H.J.M. and van Praagh, Dr. J.B. and Walters, Lisa |
| Faculty: | Medical Sciences |
| Date Deposited: | 06 Jun 2023 11:31 |
| Last Modified: | 06 Jun 2023 11:31 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/3548 |
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