Angelino, M.J. (2022) PDE3A and DDR2 might illuminate a path towards tumor-targeted imaging in gastro-intestinal stromal tumors (GISTs). thesis, Medicine.
Full text available on request.Abstract
Purpose: Gastro-intestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the gut. Surgical resection without tumor rupture and with negative surgical margins is the mainstay of treatment, but tumor rupture rates vary from 1-27 % in resections. The purpose of this study was to identify suitable biomarkers for tumor-targeted image-guided surgery. Method: Five potential biomarkers were selected through combining multiple resources (Euretos, Open Targets, Clinical Trials and a paper describing the proteomics of GISTs) and a recent article on IGS in sarcoma’s. Immunohistochemical expression of biomarkers was scored in tumor tissue and adjacent healthy tissue in a cohort of GISTs (n=23). Biomarkers were assessed using an adapted tumor selection criteria (TASC) score. Results: The selected biomarkers (CD243, PIK3CG, PDE3A, DDR2, TEM1) showed expression in respectively 70 %, 70 %, 100 %, 100 %, and 48 % of the GIST specimens. The mean staining score was 3,2 (±2,3) for CD243, 2,7 (±2,1) for PIK3CG, 6,0 (±0,9) for PDE3A, 5,3 (±1,1) for DDR2 and 2,1 (±2,4) for TEM1. No statistical difference in mean staining score was observed between DDR2 and PDE3A (p>.05) All biomarkers showed positive expression in adjacent healthy non-tumor tissue. PDE3A and DDR2 showed a high staining score and had the highest score on the basis of the adapted target selection criteria, but both showed positive expression in adjacent healthy tumor tissue. Conclusions: The results of this study show that PDE3A and DDR2 might be potential biomarkers for tumor-targeted IGS.
| Item Type: | Thesis (UNSPECIFIED) |
|---|---|
| Supervisor name: | van der Hage, prof. dr. J.A. and van Ginkel, dr. R.J. |
| Faculty: | Medical Sciences |
| Date Deposited: | 16 May 2023 12:55 |
| Last Modified: | 16 May 2023 12:55 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/3520 |
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