Bleeker, N. (Nicole) (2019) Relatie tussen parodontitis en reumatoïde artritis: Porphyromonas gingivalis gerelateerde antilichamen in klinisch gezonde controles, parodontitis patiënten en reumatoïde artritis patiënten. thesis, Dentistry.
Full text available on request.Abstract
Introduction: periodontitis is a disease which causes the destruction of the periodontium. Rheumatoid arthritis (RA) causes deterioration of the cartilage and underlying bone. These diseases reinforce each other in terms of prevalence and symptoms. The underlying link between these diseases could be the periodontal pathogen Porphyromonas gingivalis (P. gingivalis). The peptidyl arginine deiminase enzyme (PPAD) and gingipains (RgpA, RgpB en Kgp) of P. gingivalis contribute to the virulence of P. gingivalis and citrullination of proteins. These citrullinated proteins could induce anti-citrullinated protein antibodies (ACPAs), which cause rheumatoid arthritis (RA). The aim of this research is to clarify the role of PPAD and gingipains in the pathogenesis of periodontitis and RA, so that in the future there can be focused on the treatment of PPAD and gingipains to prevent periodontitis and RA and/or reduce severity. The research questions of this research are: what are the differences in concentrations anti-PPAD, anti-RgpA, anti-RgpB and anti-Kgp IgG antibodies between the groups healthy controls, periodontitis patients and rheumatoid arthritis patients with periodontitis and what is the correlation between these antibodies and Periodontal Inflamed Surface Area (PISA) and ACPAs within these patient groups. As a substudy, the same is investigated for anti-whole P. gingivalis lgG antibodies and its difference in concentrations between periodontitis patients with and without subgingival P. gingivalis, respectively Pg+ and Pg-, to ensure that these antibodies are indeed related to an infection with P. gingivalis. Material and methods: the healthy controls (HC, n=34), periodontitis patients (PD, n=42) and RA patients with periodontitis (RA, n=14) were selected out of previous research based on the availability of the following information: PISA scores, concentrations ACPAs, anti-P. gingivalis lgG antibodies and known presence or absence of subgingival P. gingivalis. The concentrations anti-PPAD, anti-RgpB and anti-Kgp lgG antibodies in the serum were measured with enzym-linked immunosorbent assay (ELISA). The statistical analysis was performed by using the one-way ANOVA, two-sided t-test and Spearmans correlation with significance level α=0,05. Results: the concentrations anti-gingipains lgG antibodies did not differ among the patient groups. The concentrations anti-PPAD lgG antibodies were lower in RA than HC (p<0,05). There were correlations in the PD group between anti-RgpB lgG antibodies and PISA score (r=0,33, p<0,05) and between anti-Kgp and anti-PPAD lgG antibodies and ACPAs (respectively, r=0,38 and r=0,32, p<0,05). In the HC group a correlation between anti-Kgp lgG antibodies and ACPAs (r=0,34, p<0,05) was found. Moreover, the concentrations anti�whole P. gingivalis lgG were higher in PD and RA than HC (respectively p<0,05 and p<0,01) and corresponding antibodies were higher for periodontitis patients Pg+ than Pg- (p<0,001). There was no correlation found between anti-whole P. gingivalis lgG antibodies with PISA and ACPAs. Discussion and conclusion: the concentrations antibodies, which were subjected in this research, between the three groups substantiate a role of P. gingivalis in the pathogenesis of periodontitis and RA. However, this cannot solely be explained by looking at PPAD and gingipains. Still, there were found weak positive correlations between anti-PPAD, anti-RgpB and anti-Kgp IgG with PISA scores and ACPAs in the groups HC and PD. Therefore, it is concluded that further research is required, before solely focusing on the treatments with respect to PPAD and gingipains to prevent periodontitis and RA.
| Item Type: | Thesis (UNSPECIFIED) |
|---|---|
| Supervisor name: | Westra, dr. J. and Smit, dr. M.J. de and Abbas, prof. dr. F. |
| Faculty: | Medical Sciences |
| Date Deposited: | 24 Nov 2021 08:41 |
| Last Modified: | 24 Nov 2021 08:41 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/2860 |
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