Koole, M. (2019) Nanoparticle and dendrimer diffusion into thick S. mutans UA159 and dental plaque biofilm. thesis, Dentistry.
Full text available on request.Abstract
Background: Caries is one of the most common diseases in dentistry. One approach to reduce risk for caries is to chemically fight oral biofilms. Diffusion into biofilms is hard because of the exopolysaccharide matrix present in the biofilm. Nanoparticles and dendrimers are small particles that have been used in different studies as carriers for antimicrobial agents. Studies about diffusion into thick cariogenic biofilms are missing. Aim: The aim was to study the role of size, surface charge and time of exposure to the fluorescent nanoparticle or dendrimer in the maximum diffusion into single species S. mutans UA159 biofilm or multi species dental plaque biofilm. Materials and methods: This randomized controlled in vitro laboratory study grew single species S. mutans UA159 biofilm and multi species dental plaque biofilm. Biofilm thickness was measured with OCT microscopy then exposed to different sized and loaded dendrimers or nanoparticles. After exposure, biofilms were frozen in a resinous compound, cryo�sectioned and examined under a fluorescence microscope. Fluorescence microscope images were analysed in a customized software and the diffusion ratio was calculated. A 3-way ANOVA was conducted to analyse biofilm diffusion. Results: One hundred and eighty-four biofilms were included in this study. The mean biofilm thickness of the S. mutans was 541.24 nm (± 123.11 nm) and for the dental plaque 217.33 nm (± 48.57 nm). There was no statistical difference in three- or two-way interaction between the three independent variables on the diffusion ratio. There was a statistically significant effect of dendrimer and nanoparticle size on the diffusion ratio where the 5 nm particles diffused the most, followed by 100 nm particles and 50 nm particles. Conclusion: Particle size plays a significant role on diffusion of nanoparticles and dendrimers into single species S. mutans UA159 and multi species dental plaque. The 5 nm particles diffused the most, followed by the 100 nm and 50 nm. Compositional features of each biofilm should be taken into consideration when evaluating any phenomena in different biofilms. Further research could focus on more repetitions, the individual characteristics of each biofilm and a wider range of nanoparticles sizes and exposure times.
| Item Type: | Thesis (UNSPECIFIED) |
|---|---|
| Supervisor name: | Miura Sugii, drs. M. and Sharma, dr. P.K. |
| Faculty: | Medical Sciences |
| Date Deposited: | 24 Nov 2021 08:07 |
| Last Modified: | 24 Nov 2021 08:07 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/2854 |
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