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Faculty of Medical Sciences

Heeft stevioside een anti-cariogene werking? Een in-vitro studie

Bode, T. (Thomas) (2018) Heeft stevioside een anti-cariogene werking? Een in-vitro studie. thesis, Dentistry.

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Introduction In addition to a non-cariogenic effect, sugar substitutes can also provide an anti-cariogenic effect. This means that fermentation of cariogenic sugars to lactate by caries-associated bacteria takes place less in the presence of this sugar substitute. This results in a lower pH drop after exposure to these fermentable sugars. Contradictions are present about the safety of synthetic sugar substitute like aspartame and saccharin. Interest in the non-caloric sweeteners extracted from leaves of Stevia rebaudiana Bertoni called stevioglycosides is increasing. The most common stevioglycoside is stevioside of which is known it acts non-cariogenic. However, little is known about its possible anti�cariogenic properties. The aim of this research is to analyze whether stevioside can act as an adequate anti-cariogenic supplement to the diet. This research’s objective is to describe the in vitro effect of various concentrations of stevioside in combination with sucrose on growth, the percentage of living cells and the lactate production of Streptococcus mutans and the pH of the growth medium. Materials and methods The anti-cariogenic effect of stevioside was determined by assessing the effect of different concentrations of stevioside (0%, 0,5%, 1% en 1,5% (w/v)) in combination with sucrose (0% en 0,5% (w/v)) on the growth and acid formation of S. mutans. Four variables were used to asses this possible anti-cariogenic effect. These concerned, after growth of S. mutans in the main cultures for 15 hours: the pH of the growth medium, the amount of the lactate produced by S. mutans, the total concentration of bacteria and the number of living / dead bacteria. The research was carried out in 20-fold. Results Addition of 1,0% (w/v) stevioside to BHI doesn’t cause a pH-drop and lactate concentrations appear to be comparable to the control groups. Stevioside in addition to 0.5% sucrose (w/v) causes a slight increase in pH after growth and incubation and a lower number of live S. mutans. A concentration of 1.0% and 1,5% (w/v) stevioside in addition to 0,5% (w/v) stevioside causes a smaller total bacterial concentration compared to main cultures with only 0.5% sucrose added. Lactate concentrations in main cultures with stevioside as addition are more or less equal to the concentrations present in BHI + 0.5 (w / v) sucrose. Conclusion Stevioside doesn’t exhibit cariogenic properties within the limits of this in-vitro research. Non�cariogenic properties of stevioside are demonstrated. An antibacterial effect of stevioside can be observed when stevioside is added to sucrose. This antibacterial effect increases as the concentration of stevioside gets higher. Based on this research, stevioside does not appear to be an effective anti-cariogenic supplement to the diet. Follow-up research must take place to demonstrate a clinical anti-cariogenic effect.

Item Type: Thesis (UNSPECIFIED)
Supervisor name: Hoogmoed, dr. C.G. van and Ma, dr. M.S.
Faculty: Medical Sciences
Date Deposited: 23 Nov 2021 12:59
Last Modified: 23 Nov 2021 12:59

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