Leene, M.J. (Marnix) (2015) Gebruik van de (in) fertility chip voor semenanalayse na vasectomie :Kan dit microfluïdische systeem in de toekomst de gouden standaard vervangen? thesis, Medicine.
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Abstract
Introduction: Approximately 42 million couples worldwide use vasectomy as their means of birth control and family planning. After the vasectomy has been performed, these men need to use other forms of contraception until 12 weeks later their semen has been analysed. This post-vasectomy semenanalysis is carried out by laboratory technicians who use a special counting chamber and a microscope to optically check for remaining sperm cells in the ejaculate. This test is expensive and time-consuming. Twente University developed in 2011 a prototype system which could be used to perform post-vasectomy semenanalysis. The microfluidic system utilises a glass chip with a nano-sized channel etched into it. Fluids like semen can be pumped through this channel and can be analyzed at the same time. This new test method we propose could make the analysis less expensive, more reliable and could possibly be performed closer to the patients home, for example in an outpatient clinic or GP’s office. Methods: First the prototype was calibrated using solutions with known concentrations of polystyrene beads and boar semen. Correlation between the results of the old and the new test method were evaluated. We then tested 7 semen samples from males who have had a vasectomy and analysed the results, looking for a correlation. Results: We have established the optimal flowrate and threshold for fluids to be pumped through the chip. For beads this was a flowrate of 0.25 μl/min and a threshold of 0.3 V. The coefficient of determination for beads was 0.94. We were not able to find any correlation between the results of the old and the new test method when testing boar semen or human ejaculate. Conclusion: This first prototype of the (in)fertility chip was able to detect polysterene beads, but analysis of boar- and human semen was disturbed by electromagnetic interference and sensitivity for shocks. Furthermore, the current prototype was unable to differentiate between spermcells and epithelial cells, erythrocytes and lymfocytes. Recommendations proposed in this paper will help further testing of boar and human semen in the future.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Facultair begeleider and Asselman, Dr. M. uroloog and Instelling Medisch Spectrum Twente Enschede, afdeling urolog |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 11:07 |
| Last Modified: | 25 Jun 2020 11:07 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/2711 |
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