Harst, E. van der (Emma) (2016) Transient Receptor Potential Channels and Inflammatory Breast Cancer : A novel approach to understand metastasis. thesis, Medicine.
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Abstract
Inflammatory breast cancer (IBC) is known as an aggressive type of cancer and is characterized by invasion of tumor emboli in the lymphatic system. Despite the severe multimodal treatment strategy, this disease carries a 5- and 10-year disease-free survival rate as low as 43% and 10%, respectively. Therefore, increasing understanding of the behavior of this cancer and discovery of targeted therapy is highly desirable. Transient Receptor Potential (TRP) channels are widely expressed non-selective cation channels, which play an important role in the calcium homeostasis of both excitable and non-excitable cells. In cancer, TRP channels show an increase or decrease in expression and are very likely involved in processes of tumor initiation and progression. However, TRP channels have yet to be studied in IBC. The aim of this study is to determine of TRP channel expression in IBC, and the involvement of these channels in tumor emboli formation and adhesion using selective and non-selective inhibitors and activators. Results of conventional Polymerase Chain Reactions (PCR) showed that TRP channels are widely expressed in IBC, with a unique pattern. While treatment with the selective TRPV-1 agonist capsaicin showed a difference in emboli size, treatment with non-selective TRPC antagonist progesterone caused a significant difference in emboli number, size, morphology, and invasive capabilities. These results suggest a role for TRP channels in the metastatic behaviour of IBC, but further research is needed to confirm this hypothesis and determine the involved molecular pathways.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Dutch Supervisor: and Schröder, Carolien |
| Supervisor name: | Direct supervisors: and Golen Kenneth L. van and and Duncan, Randall L. and University of Delaware and Center for Translational Cancer Research. Helen F. Graham Ca and Center, Cristiana Care. |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 11:07 |
| Last Modified: | 25 Jun 2020 11:07 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/2670 |
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