The Effect of the β-Human Chorionic Gonadotropin-Related Peptide (EA230) on Brain Death Induced Inflammation in Rats

Erp, A.C. van (2013) The Effect of the β-Human Chorionic Gonadotropin-Related Peptide (EA230) on Brain Death Induced Inflammation in Rats. thesis, Medicine.

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Abstract

Organ transplantation has become the treatment of choice for patients with end stage organ failure. However, the shortage of donor organs has limited the success of transplantation. Donation after brain death (DBD) is the main source of organs for transplantation. Brain death (BD), however, negatively affects organ quality and function due to hemodynamic, hormonal and inflammatory changes. We hypothesized that EA230, an oligopeptide of the β-loop of human chorionic gonadotropin (hCG), would attenuate BD-induced inflammation and improve kidney- and liver damage in an already established BD-model in rats. Plasma creatinine, ASAT, total bilirubin and IL-6, as well as renal and hepatic gene expression levels of IL-6 and MCP-1, were significantly higher in BD rats compared to sham-operated rats. Polymorphonuclear (PMN) influx in kidney and liver interstitium was also significantly higher in BD rats than sham animals. No significant differences were found between EA230- and PBS-treated animals in plasma levels of creatinine, AST, ALT, LDH, direct and total bilirubin and IL-6, in gene expression levels of IL-6, TNF-α and MCP-1, or PMN influx in the kidney or liver. In vitro results show that pre- or post-treatment of LPS-activated rat leukocytes do not affect IL-6 levels. Based on these results, we suggest that the ineffectiveness of EA230 might be related to the systemic changes related to BD in our rat model.

Item Type:	Thesis (Thesis)
Supervisor name:	Rebolledo, R.A. and Veldhuis, S. (Suzanne) and Leuvenink, Henri G. and Ploeg, Rutger J.
Supervisor name:	Ottens, Petra J. and Ploeg, Rutger J. and Department of Surgery and University of Oxford, Oxford
Faculty:	Medical Sciences
Date Deposited:	25 Jun 2020 11:07
Last Modified:	25 Jun 2020 11:07
URI:	https://umcg.studenttheses.ub.rug.nl/id/eprint/2659

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