Wullink, Bart (2012) Collageen type VII in het humane oog: structurele verbindingen : Onderzoek naar de distributie en locatie van intra-oculair collageen type VII, met name in het corpus ciliare. thesis, Medicine.
Full text available on request.Abstract
Purpose: To investigate the distribution and localisation of type VII collagen (Col VII) in the human eye, with special interest in the ciliary body. Methods: Human donor eyes were obtained from the Corneabank, Beverwijk. The postmortem time did not exceed 48 hours. These eyes were embedded in paraffin and Epon or dissected for Western blotting. Sections were cut and stained immunohistochemically (IHC) using mouse monoclonal and rabbit polyclonal primary antibodies against Col VII, according to appropriate protocols for corresponding analyzing techniques. These techniques were light- and transmission electron microscopy (LM and TEM). Secondary antibodies were chosen accordingly. For TEM technique, gold labeled secondary antibodies were used. Western blots were performed on pooled homogenized ocular substructures of ciliary body, choroid, lens capsule with zonules and retina with ILM. These substructures were derived from the donor eyes by postmortem dissection. Setting: laboratory Background: Recently, type VII collagen has been demonstrated in the human retina. The function of this type of collagen - at least in skin - is to mechanically secure tissue layers to each other. The fashion in which ocular tissue layers are fastened has not yet been elucidated.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Los, Dr. LI oogarts Oogheelkunde en BioMedical Engineering |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 11:06 |
| Last Modified: | 25 Jun 2020 11:06 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/2634 |
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