Meurs, S.J. van (2012) Antiseptics and the Prevention of Surgical Site Infections; an in vitro investigation. thesis, Medicine.
Full text available on request.Abstract
Objective: The aim of this study was to analyze the in-vitro properties (cytotoxic and bacteriostatic effect) of commonly used antiseptics and their respective dilutions. We will use this data to discover a possible candidate for intra-operative wound irrigation. The final goal is to reduce post-operative infections via this method. Methods: Bactericidal effect of antiseptics was investigated via standard quantitative suspension test; effect was investigated on S. Aureus and S. Epidermidis. Cytotoxic effect was investigated on human fibroblasts and mesenchymal stem cells with cell viability measured via the WST-1 metabolism assay. Results: Povidone-iodine was the only antiseptic which possessed a concentration with bactericidal capabilities against both strains of bacteria whilst there was still some cell vitality left. Chlorhexidine could not be investigated properly on a monolayer of cells due to the inability to be mixed with standard solution compounds (PBS, physiological saline). The other tested antiseptics did not possess this capability. Conclusions: Povidone-iodine is the only antiseptic with full bactericidal capabilities whilst retaining some viability of cells. Therefore povidone-iodine in a concentration of 1.4 g/L is in this investigation the best option to use as on intra-operative irrigation antiseptic.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Supervisor: and Kruyt M. MD PhD and Orthopedic surgeon and University Medical Centre of Utrecht (UMCU), division of ort |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 11:06 |
| Last Modified: | 25 Jun 2020 11:06 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/2619 |
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