Bui, M.T. (2016) Wat is de invloed van het groeioppervlakte en/of calcium op de rode fluorescentie van biofilms. thesis, Dentistry.
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Abstract
Objective: The aim of this research is to evaluate whether the underlying material for the growth of biofilms and calcium in the growth media influences the intensity of the red fluorescence assessed with Quantitative Light-Induced Fluorescence-Digital (QLFD). Methods: Dental biofilms were initiated from human saliva on different substrates (composite, dentin and glass). The biofilms were cultured anaerobically in different growth media using a 12-well cell culture plate. Six different growth media were used in this research: 1. BMM, 2. Sucrose, 3. 1mM Ca+sucrose, 4. 1mM Ca, 5. 10mM Ca+sucrose and 6. 10mM Ca. After 10 days QLFD images were taken of the biofilms and the intensity in red fluorescence was analysed and quantified as Red/Green-ratio (R/G-ratio). Results: The R/G-ratio of biofilms grown on dentine samples increases the most after 10 days culture; 0,28. The difference is significant compared to biofilms grown on glass (p<0,00). The increase in intensity of the R/G-ratio after 10 days culture is significant for biofilms grown in media with 10mM Ca and 10mM Ca+sucrose. The difference in R/G-ratio for biofilms grown in media with 10mM is significant compared to biofilms grown in media with BMM, 1mM Ca+sucrose or 1mM Ca (p<0,05). Conclusions: The material on which biofilms grow influences the intensity of the red fluorescence measured with QLFD. 1mM Ca has no significant effect on the intensity of the red fluorescence after 10 days growth. 10mM on the other hand has a significant influence. The intensity of the red fluorescence seems to be influenced by the calcium concentration, which might in turn can be explained by the calcification of the biofilms. In order to determine the association between this phenomenon, further research need to be performed with different calcium concentrations.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Sluis, dr. L.W.M. van der |
| Supervisor name: | Dijkstra, R.J.B. |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 11:06 |
| Last Modified: | 25 Jun 2020 11:06 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/2616 |
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