Chemokine production of buccal epithelial cells in term and preterm infants in Curaçao

Stahlie, E. (Emma) (2015) Chemokine production of buccal epithelial cells in term and preterm infants in Curaçao. thesis, Medicine.

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Abstract

Background: Previously, it has been reported that the buccal epithelial cells (BECs) of a term infants are hyperresponsive to microbial stimulation at birth. Within the first days after birth BECs achieve a hyporesponsive state. This transition, called sensibilisation, was presumably associated with colonization of the intestinal tract, leading to microbial exposure. Necrotizing enterocolitis (NEC), a disease affecting premature infants, is associated with an abnormal microbial growth of the intestinal tract. Objective: We set out to investigate whether premature infants go through the same transition as term infants in the first few days after birth. Secondary objective was to investigate whether there was an association between the interleukin-8 (CXCL-8) production of BECs and the development of NEC in later life. Design: Buccal epithelial cells were collected from term infants and premature infants. The cells were either stimulated with lipopolysacharide (LPS) or left unstimulated. We subsequently measured the CXCL-8 production. Results: Buccal epithelial cells of premature infants, as well as term infants, did not produce CXCL-8 spontaneously; neither did to they respond to microbial stimulation. The cells were therefore hypo responsive at birth. During the study, no infants with NEC were included, therefore there are no results regarding the influence of NEC on chemokine production of buccal epithelial cells. Conclusions: We show that buccal epithelial cells are hypo responsive when born and are unable to produce chemokines even in the presence of microbial stimuli or during infection.

Item Type:	Thesis (Thesis)
Supervisor name:	Supervisor: and MD, J. Hol
Supervisor name:	Second supervisor: and Hulscher J.B.F. MD, PhD and Location: and St. Elisabeth Hospital on Curaçao, Paediatric Ward
Faculty:	Medical Sciences
Date Deposited:	25 Jun 2020 11:06
Last Modified:	25 Jun 2020 11:06
URI:	https://umcg.studenttheses.ub.rug.nl/id/eprint/2609

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