Distinct DNA methylation patterns in severe intrauterine growth restricted pregnancies.

Glas, E. (Evelien) (2015) Distinct DNA methylation patterns in severe intrauterine growth restricted pregnancies. thesis, Medicine.

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Abstract

Aims. Intrauterine growth restriction (IUGR) implies many short-and long term health problems and the main cause is placental pathology. DNA methylation differences in the placenta have proven associations with IUGR. This study aims to search for DNA methylation differences for genes with a suspected influence on IUGR. Methods. 42 patients were included, groups were divided in IUGR with birth weight <p2.3 (n=15), IUGR with birth weight between p5-p10 (n=15), and a control group (n=12). After literature search, PlGF, SPRY2, ADAM12, TBX15 and HSD11B2 were selected as research genes. For measuring methylation differences, pyrosequencing was performed on isolated DNA from paraffin-embedded placental tissue. Results. A significant hypomethylation of PlGF between the IUGR p<2.3 and control group is seen at CpG position 2 (p=0.008) and at position 4 (p=0.038) between the p<2.3 group and p5-p10 group. The other genes showed no reliable significant differences. Reliable pyrosequencing results on paraffin-embedded placental material was generated. Conclusion. Hypomethylation of PlGF is associated with IUGR pregnancies with a birth weight under the p2.3. More research according the biological background op PlGF should be performed for better understanding. Secondary, this study proved it possible to perform reliable methylation studies on paraffin-embedded placental tissue.

Item Type:	Thesis (Thesis)
Supervisor name:	Eijsink, Dr. J.J.H. and Scherjon, Prof. dr. S.A. and Plösch, Dr. T.
Faculty:	Medical Sciences
Date Deposited:	25 Jun 2020 11:05
Last Modified:	25 Jun 2020 11:05
URI:	https://umcg.studenttheses.ub.rug.nl/id/eprint/2476

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