Krakers, M. (2015) Immunogenicity of the Bacterial Endonuclease MazF in CD4+ Lymphocytes Retrovirally transduced to express MazF in HIV-treatment. thesis, Medicine.
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Abstract
3 1.1 Summary Background: One novel therapy to treat HIV is to transduce CD4+ lymphocytes with the bacterial endonuclease MazF, MazF being regulated by the Tat protein . When HIV infected CD4+ cells are transduced with MazF, it reduces viral replication and viral fitness of HIV without having negative effects on cell growth and viability. What is however unknown, is if there is an immune response to MazF when patients are infused with MazF induced CD4+ lymphocytes (MazF-T-cells). Study: In order to determine this, we performed functional polychromatic flow cytometry on samples from patients that were infused with MazF-T-cells and also on non-experimental treatment for HIV. In this case HAART. These samples were collected at nine different time points (from before infusion until up to 1.5 years) to measure the possible T cell response over time with polychromatic flow cytometry. These samples were stimulated for 6 hrs with peptide pools, consisting of 15-mers, overlapping by 11 amino acids, covering the MazF open reading frame to determine if this would trigger cytokine release and degranulation by T cells. Results: In none of the samples from this patient cohort an anti-MazF T cell response was detectable. Conclusion: Though MazF is most likely immunogenic, we did not detect a T cell response against this bacterial protein.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Faculty Supervisor: and Kallenberg, Prof. dr. C.G.M. |
| Supervisor name: | Second Supervisor: and Melenhorst, Dr. J.J. and Location: Translational and Correlative Studies Laboratory |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 11:05 |
| Last Modified: | 25 Jun 2020 11:05 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/2468 |
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