Sloot, E. van der (Emma) (2015) The effects of tetrahydrobiopterin in combination with tyrosine and tryptophan supplementation on cerebral monoamine neurotransmitter concentrations in a PKU mouse model. thesis, Medicine.
Full text available on request.Abstract
We investigated the effects of tetrahydrobiopterin and tyrosine and tryptophan supplementation on neurotransmitter concentrations in a mouse model of the congenital metabolic disease phenylketonuria. This was done using control groups for the tetrahydrobiopterin treatment, as well as for the amino acid supplementation, and for the mice having the disease-causing mutation, as for their wild type littermates. The treatment was administered for 4 weeks. After termination, we investigated brain neurotransmitter and amino acid concentrations. We hypothesized that concentrations of serotonin and catecholamines in the brain would increase, when tetrahydrobiopterin is administered, as well as increasing precursor availability for neurotransmitters, by supplementation of the amino acids tyrosine and tryptophan. This could be a step towards improving outcomes and possibly making less strict dietary regimens possible. The results of our current study indicate that the used dosage of BH4 did not have an effect on neurotransmitter synthesis. There were no differences between BH4 and placebo treated groups. However, increased neurotransmitter concentrations were found in mice on the tyrosine and tryptophan enriched diet. Follow-up studies should address whether higher BH4 dosage is able to improve neurotransmitter synthesis and if this should be combined with a phenylalanine restricted diet
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Spronsen, Prof. Dr. F.J. van and Vliet, D. van |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 11:02 |
| Last Modified: | 25 Jun 2020 11:02 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/2259 |
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