Visscher, G. (2012) Efficacy of Whole Body Vibration therapy in OIM mice : An analysis of the effect of WBV on Tibial Trabecular Bone Formation and Serum Bone Remodeling markers in OIM mice. thesis, Medicine.
Full text available on request.Abstract
Background: WBV study The OIM mouse model (B6C3fe-a/a-oim) mimics intermediate severe osteogenesis imperfect (OI) in human. OIM mice have severe osteopenia, bone fragility and multiple fractures. High frequency low magnitude whole body vibration (WBV) can increase bone density or prevent the loss of bone. This study was performed to examine the effect of WBV on OIM bone, analyzing trabecular bone formation and bone remodeling markers. Pilot-study Mesenchymal stemcells transplanted in OIM mice in utero (OIM+IUT) gave a significant change in fracture rate (≥70%) albeit bone engraftment rates were very low (≤5%). In this pilot study, the quantity of human collagen in humeri of OIM+IUT mice was analysed. Methods: WBV study Homozygous B6C3Fe a/a-Col1α2oim/Col1α2oim (OIM, 5 male, 5 female) mice and wild type B6C3Fe a/a-Col1α2+ /Col1α2+ (WT, 5 male, 5 female) were subjected to whole body vibration (WBV) for 15min/day, 5 days/week at 45Hz, 0.3g. Control WT (WT sham, 5 male, 5 female) and OIM (OIM sham, 5 male, 5 female) were put on a non-vibrating platform (15min/day, 5days/wk) or got no treatment. In the proximial metaphysis of tibial bone, mineralizing surface on bone surface (MSBS), mineralized bone formation rate (MdBFR) and mineral apposition rate (MAR) were examined. Markers of bone remodeling (CTX-I reflecting bone degradation) and PINP reflecting bone formation) were quantified in serum of female WT and female OIM mice. Pilot study Collagen, extracted from humeri of WT (n = 2) , OIM (n = 2) and OIM+IUT (n = 2) was analyzed using MALDI/TOF-TOF mass spectrometry. Results: WBV study In both WT and OIM mice (pooled for sex), WBV did not give a significant change in MSBS, MAR and MdBFR compared to Sham mice. In female WT mice, WBV gave a significant increase in MdBFR of 38% (p = 0.047). In the other WT and OIM mice, no significant difference was found in MSBS, MAR and MdBFR between vibrated and sham mice in either the male or the female group. In WT mice, WBV decreased PINP absorbance levels with 20% (p = 0,02). In OIM mice, WBV lowered the absorbance level of CTX-I with 26% (p = 0,03). Pilot study Human collagen type I could not be detected in OIM+IUT mice. Conclusion : WBV study No clear evidence is found for the efficacy of WBV in improving bone formation in OIM mice. Bone remodeling markers reveal an increased bone degradation in WBV OIM mice, while bone formation does not change. However, due to limitations in the protocol used for the histological analysis of bone, actual change in trabecular bone formation may have been missed. Further research is needed to investigate the potential of WBV therapy in improving the structure of trabecular bone in OIM mice. Pilot study Albeit previous research done by Vanleente et al. showed that OIM+IUT mice produced human collagen, human collagen levels were too low to detect with mass spectrometry, implying a small quantity of collagen, however able to cause a large change in bone structure in the OIM+IUT mice. Further research is needed to evaluate the ratio between human and mice collagen in OIM+IUT mice to answer the question what such strong decline in fracture rates in OIM+IUT mice is caused by.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Supervisor (1) and Shefelbine Dr. S and Supervisor (2) and Bank Prof. Dr. RA and Co-supervisor and Vanleene, Dr. M and Department Biomedical Engineering and Imperial College London |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 11:00 |
| Last Modified: | 25 Jun 2020 11:00 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/1997 |
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