Neleman, L. (2018) Maturity and functionality of umbilical cord blood CD4+CD25+FoxP3+ cells not as heterogeneous as in healthy adult peripheral blood: an in-depth comparison of the differentiation and function of T regulatory cells. thesis, Medicine.
Full text available on request.Abstract
English: Manipulation of T regulatory cells (Treg) presents the potential for novel therapeutics. These CD4+CD25+FoxP3+ cells are essential for the maintenance of immune homeostasis, and Treg dysfunction is correlated with various immunological disorders. Due to technical restrictions, Treg research has been limited to standard resolution. Additionally, Treg research has been limited to non-human animals and adults. The introduction of mass cytometry recently allows for a high resolution analysis of Treg. Umbilical cord blood (CB) Treg have never been analyzed nor compared to adults at high resolution, hence it is unsure if heterogeneous diversity exists. As CB transplants have been grossly observed to reduce the incidence of graft versus host disease in leukemia patients, it is necessary to research CB Treg at high resolution. Specific CB Treg cluster expansion could prove to be an attractive development. Within the Cytobank database, mass cytometry acquired CB and healthy adult peripheral blood data was collected. Treg heterogeneity was analyzed with respect to phenotype, functionality, maturity and marker expression. The data revealed that CB Treg heterogeneity is already established and diverse. Still, adult Treg have higher functional and maturity diversity than CB. As heterogeneity is already established and CB Treg expressed higher intensities of markers for proliferation and immune suppression, expansion of specific CB Treg subpopulations with specific markers expression could prove to be a novel immunological therapeutic. This paper is the first to have comprehensively analyzed CB Treg using high dimensional techniques.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Faculty Supervisor: and Horvath, Dr. B. |
| Supervisor name: | Secondary Supervisor: and Matos, Dr. T. R. and Department of Dermatology, Academic Medical Center |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:59 |
| Last Modified: | 25 Jun 2020 10:59 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/1918 |
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