Laskewitz, A. (Anna) (2017) Differential distribution of immune cells in decidua basalis and decidua parietalis after uncomplicated term pregnancy, a pilot study. thesis, Medicine.
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Abstract
Introduction During pregnancy tolerance towards a fetus needs to be created by the maternal immune system to prevent a rejection response. Maladaptation of the maternal immune system is associated with pregnancy complications like preeclampsia, gestational diabetes mellitus and infertility. The placenta and fetal membranes are important locations where the maternal immune system needs to create tolerance towards the fetus. Different immune cells like Tcells and macrophages are known to be located in these tissues and found to be important during pregnancy. This study aims to analyze maternal tissue from the placenta (decidua basalis) and maternal tissue from the fetal membranes (decidua parietalis) to see whether there is a differential distribution of immune cells at these locations. Materials and methods The isolation of T-cells and macrophages consisted of a mechanical step and an enzymatic step. First, maternal and fetal tissue were separated to analyze only maternal tissue. Next, both tissues were meshed and with an enzymatic solution (Accutase) cells were separated from both tissues. Using flow cytometry, subsets of T-cells and macrophages were analyzed in decidua basalis and decidua parietalis. Decidual tissues were compared using a two-way ANOVA. Results Higher amounts of different subsets of T-cells were found in decidua parietalis as compared to decidua basalis. In particular, higher percentages of regulatory T and memory cells were found in decidua parietalis as compared to decidua basalis. A more regulatory phenotype of macrophages was found in decidua basalis as compared to decidua parietalis. Conclusion Differences between decidua basalis and decidua parietalis were found for different subsets of T-cells and macrophages. Using comprehensive and up-to-date antibody panels new knowledge about the maternal immune cells in uncomplicated term pregnancies is brought forward.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Daily supervisor and Prins, Dr. J.R. and Faculty supervisor and Faas, Dr. M.M. and Department of Obstetrics and Gynecology and Department of Pathology and Medical biology, division of and Medical Biology and University Medical Center Groningen (UMCG) |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:57 |
| Last Modified: | 25 Jun 2020 10:57 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/1796 |
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