Heidotting, J (Jeffrey) (2017) The Effects of different irrigating Solutions in Combination with different Temperatures on the Metabolism of Chondrocytes. thesis, Medicine.
Full text available on request.Abstract
Arthroscopic surgery is a common procedure in daily practice. 0.9% NaCl saline solution is the most commonly used irrigating solution during these procedures, but in vitro studies have shown a negative effect of this irrigating solution on the uptake of radio-labelled SO4, being an indicator for the amount of proteoglycan synthesis in cartilage. A lower metabolism means a longer recovery period of the cartilage after the arthroscopic procedure. Other irrigating solutions, and perhaps also different temperatures, may have potential as an alternative to irrigate cartilage. To our knowledge, different irrigating solutions at different temperature have not been tested on cartilage and chondrocytes. This study investigated the effect of three different irrigating solutions at three different temperatures (4°C, 20°C, 37°C) on the metabolism on chondrocytes. Twelve different genes were investigated, giving an impression of the proteoglycan synthesis, collagen synthesis and endoplasmic reticulum stress levels. Cartilage and chondrocytes were exposed to the experimental conditions for two hours, similar to a normal-long arthroscopic procedure. Results were compared to control medium, i.e. medium which is normally used to culture chondrocytes. No significant differences were found between irrigating solutions or between temperatures in the cartilage and chondrocyte group after 24 hours or 48 hours. This study shows that there is no need to use a specific irrigating solution at a specific temperature and that the current practice does not have an effect on chondrocyte metabolism.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Bank, Prof. Dr. R.A. |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:56 |
| Last Modified: | 25 Jun 2020 10:56 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/1705 |
Actions (login required)
 |
View Item |