Promoting the proliferation and differentiation of embryonic and stem cell derived cardiomyocytes.

Vegter, E. (Eline) (2013) Promoting the proliferation and differentiation of embryonic and stem cell derived cardiomyocytes. thesis, Medicine.

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Abstract

Cardiovascular disease is one of the world’s leading causes of mortality in developed countries. Heart failure is characterized by an irreversible loss of functional heart muscle cells. Although there are many advantages made in the current therapeutic options, unfortunately until now it is not possible to restore heart muscle function by replacing or regenerating new cardiac cells. This study in the field of cardioregenerative medicine focuses on the proliferation and differentiation of embryonic heart cells and cardiac progenitors into cardiomyocytes. In this study, an effective screening method with small molecules is set up to screen for potential stimulators of early cardiomyocyte proliferation. At least 4 compounds have been identified as promoters of cardiomyocyte expanding and further research would elucidate their working mechanisms and possibly unravel new pathways of cardiogenesis. Lineage commitment of cardiac progenitor cells to the cardiomyocyte cell line is a complicated process in which many influencing factors are involved. Here, we examined whether cell shape as a geometric cue plays a role in the differentiation of cardiac progenitors into cardiomyocytes. In this study, no difference in cardiomyocyte differentiation is seen between unpatterned and patterned cells. However, further studies are needed to confirm this finding.

Item Type:	Thesis (Thesis)
Supervisor name:	Meer, P. van der MD
Supervisor name:	Domian, I.J. MD and Harvard Medical School, Boston
Faculty:	Medical Sciences
Date Deposited:	25 Jun 2020 10:40
Last Modified:	25 Jun 2020 10:40
URI:	https://umcg.studenttheses.ub.rug.nl/id/eprint/166

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