An antifouling and biofilm inhibiting PLGA coating for biomaterials using inulin incorporated DNase I and gentamicin.

Beldman, M.M. (2014) An antifouling and biofilm inhibiting PLGA coating for biomaterials using inulin incorporated DNase I and gentamicin. thesis, Medicine.

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Abstract

Biomaterial associated infections (BAI) are a difficult to treat problem due to biofilm formation. Extracellular DNA (eDNA) found in the extracellular polymeric substance (EPS) of the biofilm has a great role in bacterial adhesion and biofilm structure. Deoxyribonuclease I (DNase I) has the capability to impair bacterial adhesion and biofilm formation. Coating biomaterials with DNase I in a controlled release system has a great potential in BAI prevention. DNase I has no bactericidal properties, so combining it with antibiotics may improve effectiveness. Stability of DNase I can be increased by incorporation of the protein in an inulin glassy matrix. In this thesis we investigated the effect of a coating of inulin incorporated DNase I, with or without gentamicin addition, in a Poly Lactic-co-Glycolic Acid (PLGA) matrix on bacterial adhesion and biofilm formation. The DNase I coating decreased adhesion and biofilm formation dramatically up to a period of 72 hours. An additional effect of gentamicin on biofilm inhibition is seen at 24 hours. Favorable release kinetics were achieved and the biomaterial was left uncovered after one week. The coating is also proven to be biocompatible. These are promising results for future clinical application of the coating.

Item Type:	Thesis (Thesis)
Supervisor name:	Sharmaa, Dr. P. K. and Jutteb, Dr. P. C. and Hinrichsc, dr. W L.J.
Faculty:	Medical Sciences
Date Deposited:	25 Jun 2020 10:55
Last Modified:	25 Jun 2020 10:55
URI:	https://umcg.studenttheses.ub.rug.nl/id/eprint/1604

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