Hegeman, M. (Margriet) (2019) Free thiols and sFlt-1/PlGF ratio as biomarkers for early onset detection of pre-eclampsia and fetal growth restriction. thesis, Medicine.
Full text available on request.Abstract
Aim: The aim of this prospective cohort study is to create a combination of noninvasive biomarkers that can be used in clinical settings in the diagnostic process of fetal growth restriction (FGR) and to extend the understanding of the pathophysiology of early onset preeclampsia (PE). Background: Oxidative stress, generated by an excess of reactive oxygen species (ROS), plays an important role in the pathophysiology of both, FGR and PE. As free thiols are easily oxidized by ROS, their circulating levels may directly reflect the level of oxidative stress. This report reflects concentration free thiols in serum and urine as noninvasive biomarker for pregnancy complications like FGR and PE. Methods: Nine patients with severe FGR, eleven patients with FGR and PE, four patients with SGA and fourteen healthy pregnant women were enrolled in the study. The concentration serum and urinary free thiols (µM) were quantified using the Ellman’s method. Results: Patients complicated with FGR and PE had significant lower concentrations serum free thiols compared to the other groups (p = 0.002). No significant difference was found between patients with FGR and other groups (p = 1.000). For concentration free thiols in 24hour and morning urine, no significant differences were found (p = 0.113, p = 0.169). Conclusion: This report showed lower concentration serum free thiols in patients complicated by PE, independent of FGR. Serum samples turned out to be more effective in the measurement of concentration free thiols, although urinary samples would be less invasive. Further investigation is needed with larger cohorts. To validate our findings, we recommend adding a patient-group with PE alone to the cohort. We also recommend delving into multiple types of biomarkers, like sFlt-1/PlGF ratio, to create a combination of noninvasive biomarkers.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Supervisors: and Gordijn, Dr. S.J. and Goor, Prof. dr. H. van and Department of Obstretics and Gynaecology Department of Patho |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:40 |
| Last Modified: | 25 Jun 2020 10:40 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/155 |
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