Cicek, A. (2010) Een pilot studie naar de microbiele diversiteit van biofilm, gerelateerd aan caries. thesis, Dentistry.
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Abstract
Biofilms in the human caries process exhibit marked diversity. PCR based denaturing gradient gel electrophoresis (DGGE) surveys microbial diversity by displaying PCR- nerated 16S rRNA fragments from micro-organisms in a biofilm24. The objective of this study was to use Denaturing Gradient Gel Electrophoresis (DGGE) to study the shift in microbial composition in the different groups: enamel versus dentine lesions, 1 week old versus 20 weeks old biofilm and primary versus secondary caries lesions. We hypothesized a microbial diversity in all of the groups, because of the differences in substrate (enamel, dentine, restored and unrestored) and the time in the caries process. We investigated the biofilm on restored and unrestored dentin and enamel samples in an in situ study in 8 subjects after 1 and 20 weeks (Thomas RZ et al., 2008). The 62 biofilm samples (originally 64, two were lost in the time) were used for DGGE analysis. Mter DNA extraction, part of the 16SrRNA gene was amplified by PCR and separated by DGGE, resulting in banding patterns roughly representing single species. Shifts in microbial diversity were determined by counting the coefficient of similarity (Cs%), Zijnge et al [2003]. A Cs% more than 85 was the norm for no differences in the microbial diversity, Zijnge et al. [2003]. Similar banding patterns and different microbial diversity were found for biofilm from enamel versus dentine lesions and primary versus secondary caries lesions. The coefficient of similarity for enamel versus dentine lesions was 64% and for primary versus secondary lesions 79% (enamel) and 84%(dentine). Significance differences were found in the banding patterns from 1 week old compared to a 20 weeks old biofilm (P=0.03) with a low coefficient of similarity (24% ). These findings suggest that there is a microbial diversity between enamel versus dentine lesions, 1 week old versus 20 weeks old biofilm and primary versus secondary caries lesions. Further investigation is needed to detect the micro-organisms which determines the differences in the microbial diversity.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Begeleider: and Thomas, Dr. R.Z. and Afdeling Conserverende Tandheelkunde and Discipline Groep Tandheelkunde/ Mondzorgkunde. and Faculteit der Medische Wetenschappen, Rijksuniversiteit Gron and Mede begeleider: and Zijnge, Dr. V. |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:54 |
| Last Modified: | 25 Jun 2020 10:54 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/1490 |
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