Hartveld, L. (Loes) (2017) The utilisation of autologous, allogeneic or human red blood cells for normothermic machine perfusion of ischemically damaged porcine kidneys. thesis, Medicine.
Full text available on request.Abstract
Objectives: the first part of this study considered the cell number, migration characteristics, and excretion dynamics of mesenchymal stem cells (MSCs) after administration to isolated porcine kidneys by analysing functional and morphological aspects of the kidneys preserved by normothermic machine perfusion (NMP). The second part of this study focused on whether the utilisation of RBCs from either autologous, allogeneic or human sources damages the isolated porcine kidney during NMP. Methods: porcine kidneys (n = 5 in each group) were subjected to a warm ischemic period of 30 minutes and then preserved by hypothermic machine perfusion (HMP) for approximately 1.5-2 hours. Next, the ischemically damaged porcine kidneys were preserved by NMP for an additional 7 hours. After 1 hour of reperfusion 1.0 x 107 MSCs derived from adipose tissue (AT) or bone marrow (BM) were administered to the NMP system (experimental group 1 and 2). Porcine kidneys were reperfused with a leukocyte-depleted, washed RBC-based solution from either allogeneic or human RBCs (experimental group 3 and 4). During the experiments, several samples of perfusate and urine were collected hourly. Tissue biopsies were taken at certain time point. Perfusion parameters were documented throughout. Results: experiments involving MSCs were cancelled due to the appearance of contamination upon cell culturing. Cumulative diuresis, creatinine clearance, urinary albumin and glucose, and cell injury markers were significantly higher in the human RBC group, suggesting severe damage. Conclusions: the use of human RBCs during NMP of an isolated porcine kidney does lead to additional injury in comparison with autologous or allogeneic RBCs. Allogeneic RBCs do not seem to damage the porcine kidneys more than autologous RBCs do. In order to overcome cell medium contamination, the culture-expanded MSCs that reached confluence that are still at low passage should be administered instantly during NMP. Therefore, the culturing of MSCs needs to be planned very well.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Leuvenink, Prof. dr. Henri G.D. and Faculty Supervisor and Moers, Dr. Cyril and Daily Supervisor and University Medical Center Groningen and Department of Surgery – Surgical Reaearch Laboratory |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:54 |
| Last Modified: | 25 Jun 2020 10:54 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/1466 |
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