Paneth cells in the developing gut: when do they arise and when do they become immune competent?

Beyduz, G. (Gaia) (2015) Paneth cells in the developing gut: when do they arise and when do they become immune competent? thesis, Medicine.

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Abstract

Introduction: Extremely-low birth weight human infants generally have the longest postnatal interval before the onset of necrotizing enterocolitis (NEC). Several authors have connected the development ofNEC with Paneth cell (PC) development. Little is known about the temporal development of PCs during gestation. Therefore we aimed to investigate the development of PCs in time during gestation. Material and Methods: Archival ileocecal resection specimens derived from autopsies from embryos, fetuses and infants ranging from a gestational age (GA) of 9 - 40 weeks. Patients were without abdominal disease. Hematoxylin-eosin (HE) staining and anti-human defensin 5 (anti-HD5) immunohistochemistry was performed to investigate when PCs arise and when they become immune competent. A semi-quantitative assessment of (immune competent) PCs abundance per 10 crypts per tissue section was performed and scored blinded by two trained observers. Results: We included 57 patients in this study. PC abundance (PCa) and quantity of immune competent PCs were associated with GA (HE: rho=0.41, p=0.002; anti-HD5: rho=0.61, p=<0.001). Immune competent PCa was significantly higher from 29 weeks GA (anti-HD5: median 6, range 0 - 18) compared to infants less than 29 weeks GA (anti-HD5: median 2, range 0-9, p<0.001). PCa increased even further after the 37th week of gestation (HE: median 7, range 0 - 12; anti-HD5: median 10, range 3 - 18) compared to preterm infants (HE: median 0, range 0 - 15, p=0.002; anti-HD5: median 4, range 0-17, p=0.003). Conclusion: This study represents the first of its kind to investigate the development and functioning of PCs per GA in human tissue. The significant increase of immune competent PCs starting at GA of 29 weeks mimics the rise in incidence of NEC over the same temporal span.

Item Type:	Thesis (Thesis)
Supervisor name:	Hulsher, Dr. J.B.F. and Heida, Dr. F.H. and Timmer, Dr. A.
Faculty:	Medical Sciences
Date Deposited:	25 Jun 2020 10:53
Last Modified:	25 Jun 2020 10:53
URI:	https://umcg.studenttheses.ub.rug.nl/id/eprint/1418

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