Heeg, E. (2015) Imaging microcirculation with optical techniques in a tissue-like flow phantom. thesis, Medicine.
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Abstract
Introduction: During surgery, access to information about the vascular circulation is essential. With this information a surgeon could earlier anticipate a vascular compromise, and decrease the chance of complications. There is no consensus on which technique should be used for imaging and quantifying microvasculature in human tissue. The aim of this study is to test the feasibility of OCT, SDF, IDF and LSCI in imaging microcirculation and in measuring perfusion parameters in a controlled laboratory setting. Furthermore, we try to report which technique comes closest to characteristics of an ideal optical method for imaging microvasculature. Methods: We made three tissue-like flow phantoms with channels of 400, 100 and 50 m in diameter at different depths and perfused these with blood at different pump flow velocities. We imaged blood flow with OCT, SDF, IDF and LSCI and quantified vessel diameter, flow velocity and maximum measuring depth. Results: OCT could image blood flow in all channels up to a depth of 1.5 mm, however underestimates vessel diameter of the 400 m channel and overestimates the 100 and 50 m channel. SDF and IDF could image blood flow in the 100 and 50 m channel up to 522 m in depth, but not in the 400 m channel. IDF constantly overestimates vessel diameter in the 100 and 50 m vessel. SDF overestimates channel diameter in the 100 m channel, but only underestimates in the 50 m channel. LSCI could only image blood flow and showed a relative flux increase when increasing pump flow velocity in the 400 m channel. Conclusion: All four optical techniques are capable of imaging blood flow, however blood flow in the smaller channels could not be imaged and quantified with LSCI. Measured blood flow velocity in smaller channels seems to be unreliable with SDF and IDF, because of pressure artifacts.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Stenekes, M.W. M.D.Ph.D. |
| Supervisor name: | Jansen, S.M. MD and Strackee, S.D. M.D.PhD and Bruin, D.M. De PhD and Academic Medical Centre and University of Amsterdam |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:50 |
| Last Modified: | 25 Jun 2020 10:50 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/1139 |
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