Lanting, G.D. (Geert) (2018) Unravelling Incomplete Systemic Lupus Erythematosus B- and T-cell subsets in incomplete SLE and SLE. thesis, Medicine.
Full text available on request.Abstract
Introduction The diagnosis of Systemic Lupus Erythematosus (SLE) is primarily based on the ACR-criteria, of which at least 4 of the 11 are required. Incomplete-SLE (iSLE) is defined as having at least 2 but less than 4 criteria. About 10-55% of iSLE patients progress to SLE within 5-10 years. Currently, there are no predictive markers which can predict progression from iSLE to SLE. However, evidence suggests several changes in the innate and adaptive immune system are occurring prior to and during development of SLE. Therefore the aim of this study was to investigate differences in B- and T-cell subsets in iSLE compared to healthy controls (HC) and SLE-patients at group level. Ultimately identifying those iSLE patients who progress to SLE. Methods 37 iSLE, 39 SLE and 12 HC were compared using fluorescence-activated cell sorting (FACS) analysis acquired at baseline. B- and T-cell subsets were identified by phenotypical analysis. Results T-cell analysis showed a decrease of lymphocytes and CD4+ T-helper cells and a shift in memory T-cell subset distribution to Effector Memory in SLE vs. iSLE. No differences were found among T-helper 17 and regulatory T-cells at group level. Regarding B-cells, results showed increased double negative B-cells and decreased non-switched memory B-cells in both iSLE and SLE vs. HC. No differences were found in memory, plasmacells/plasmablasts, transitional and Age-associated B-cells (ABC’s). T-bet was lower in total B-cells in SLE vs. HC and lower in ABC’s in SLE and iSLE vs. HC. Conclusion Few significant differences were found within the T-cell subsets. Significant differences were found within double negative and non-switched B-cells next to a shift within T-bet expression, which make these subsets interesting candidates look at in follow-up studies and to see if they can be used to identify those iSLE patients who progress to SLE.
| Item Type: | Thesis (Thesis) |
|---|---|
| Supervisor name: | Under supervision of and Leeuw, Dr. K de and Westra, Dr. J. and Department of Rheumatology and Clinical Immunology and University Medical Centre Groningen |
| Faculty: | Medical Sciences |
| Date Deposited: | 25 Jun 2020 10:49 |
| Last Modified: | 25 Jun 2020 10:49 |
| URI: | https://umcg.studenttheses.ub.rug.nl/id/eprint/1052 |
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